IFG

In vitroevaluation of the antimicrobial properties of Mallotus oppositifoliumdecoction leaf extracts and fractions

Roger ZINTCHEM1, Gaëtan Olivier FANKEM1, Charles FOKUNANG2*, Pierre MKOUNGA3, Estella Achick TEMBE2, René KAMGANG1, Donatien GATSING4 and Théophile DIMO1

1Department of Biology and Animal Physiology, P.O. Box 812 Yaoundé, Cameroon.
2Department of Pharmacotoxicology and Pharmacokinetics, P.O. Box 33032 Yaoundé, Cameroon.
3Department of Organic Chemistry, P.O. Box 812 Yaoundé, Cameroon.
4 Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67 Dschang, Cameroon.
*Corresponding author: E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. ; +237 670902446.

ABSTRACT

In order to verify the in vitro antimicrobial properties of Mallotus oppositifolium (Euphorbiaceae), the qualitative phytochemical screening and the antimicrobial activities on Shigella dysenteriae A2, Salmonella typhi, Escherichia coli, Enterococcus faecalis, Staphyllococcus aureus and Candida albicans strains of the aqueous decoction (DEMO) hexane (HEMO) and methanol (MEMO) fractions of leaves were assessed. The screening was performed using colorimetric methods. The antimicrobial activity was carried out using disc diffusion assays. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by the dilution methods. The screening revealed the presence of metabolites such as alkaloids, flavonoids, phenolic compounds, tannins, saponins, lipids, carbohydrates, mucilages, proteins, triterpens and steroids. DEMO, HEMO, MEMO showed a concentration-dependent activity against Shigella dysenteriae A2 and Salmonella typhi, with inhibition zone ranging from 9.44±0.44 to 19.00±0.24 mm, 8.94±0.05 to 20.03±0.17 mm and 8.13±0.17 to 16.76±0.11 mm respectively. The MIC showed ranges from 0.25 to 1.00 mg.mL-1, 0.20 to 0.50 mg.mL-1 and 1.00 to 3.00 mg.mL-1, while the MBC ranged from 1.00 to 5.00 mg.mL-1, 0.50 to 1.00 and 3.00 to 10.00 mg.mL-1 respectively. The leaves decoction and fractions activity on the two strains showed promising activities to justify the use of the plant against diarrhoea in folk medicine.

Keywords: Folk medicine, phytochemical analysis, Mallotus oppositifolium diarrhea, antimicrobial.

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